XRN2 Mouse Monoclonal Antibody [Clone ID: OTI5F4]

CAT#: CF809427

Carrier-free (BSA/glycerol-free) XRN2 mouse monoclonal antibody,clone OTI5F4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY XRN2 (Cat# RC223980, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRN2 (Cat# TA809427)(1:2000). Positive lysates LY415881 (100ug) and LC415881 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-XRN2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-XRN2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809427) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5F4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 677-779 of human XRN2(NP_036387) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 108.4 kDa
• Gene Name: Homo sapiens 5'-3' exoribonuclease 2 (XRN2), transcript variant 2, mRNA.
• Database Link:
  NP_036387
  Entrez Gene 24128 MouseEntrez Gene 22803 Human
  UniProt ID Q9H0D6
• Background: This gene shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The yeast gene is involved in homologous recombination and RNA metabolism, such as RNA synthesis and RNA trafficking. Complementation studies show that Dhm1 has a similar function in mouse as dhp1. The function of the human gene has not yet been determined. Transcript variants encoding different isoforms have been noted for this gene; however, their full-length nature is not known. [provided by RefSeq, Jul 2008]
• Synonyms: 5'-3' exoribonuclease 2; Dhm1-like protein (mouse homolog); OTTHUMP00000030403

Reference Data

• Protein Pathways: RNA degradation