MGMT Mouse Monoclonal Antibody [Clone ID: OTI2F10]

CAT#: CF809131

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Carrier-free (BSA/glycerol-free) MGMT mouse monoclonal antibody, clone OTI2F10 (formerly 2F10)



  View other "OTI2F10" antibodies (4)

USD 600.00


Availability*
In Stock

Size
    • 100 ug


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Specifications

Product Data
Clone Name OTI2F10
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human MGMT (NP_002403) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT), mRNA.
Background Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]
Synonyms methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741
Reference Data
Protein Families Druggable Genome

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