SHMT2 Mouse Monoclonal Antibody [Clone ID: OTI3E9]

CAT#: CF808820

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Carrier-free (BSA/glycerol-free) SHMT2 mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)

Formulation: Standard Carrier-Free



  View other "OTI3E9" antibodies (4)

USD 600.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI3E9
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human SHMT2(NP_005403) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 52.5 kDa
Gene Name Homo sapiens serine hydroxymethyltransferase 2 (SHMT2), transcript variant 1, mRNA; nuclear gene for mitochondrial product.
Background This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
Synonyms GLYA; HEL-S-51e; NEDCASB; SHMT
Reference Data
Protein Pathways Cyanoamino acid metabolism, Glycine, serine and threonine metabolism, Metabolic pathways, Methane metabolism, One carbon pool by folate

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