MAP3K8 Mouse Monoclonal Antibody [Clone ID: OTI2E10]

CAT#: CF808427

Carrier-free (BSA/glycerol-free) MAP3K8 mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP3K8 (Cat# RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8 (Cat# TA808427)(1:2000). Positive lysates LY401593 (100ug) and LC401593 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2E10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 277-461 of human MAP3K8(NP_005195) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.7 kDa
• Gene Name: mitogen-activated protein kinase kinase kinase 8
• Database Link:
  NP_005195
  Entrez Gene 26410 MouseEntrez Gene 116596 RatEntrez Gene 1326 Human
  UniProt ID P41279
• Background: This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
• Synonyms: AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, T cell receptor signaling pathway, Toll-like receptor signaling pathway