FGFBP1 Mouse Monoclonal Antibody [Clone ID: OTI2B6]

CAT#: CF808309

Carrier-free (BSA/glycerol-free) FGFBP1 mouse monoclonal antibody, clone OTI2B6 (formerly 2B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FGFBP1 (Cat# RC200819, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FGFBP1 (Cat# TA808309)(1:2000). Positive lysates LY417486 (100ug) and LC417486 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-FGFBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808309) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FGFBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808309) (1:150)

Specifications

Product Data

• Clone Name: OTI2B6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 24-234 of human FGFBP1(NP_005121) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: fibroblast growth factor binding protein 1
• Database Link:
  NP_005121
  Entrez Gene 9982 Human
  UniProt ID Q14512
• Background: This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 4. [provided by RefSeq, Nov 2011]
• Synonyms: FGF-BP; FGF-BP1; FGFBP; FGFBP-1; HBP17

Reference Data

• Protein Families: Secreted Protein