NDUFB11 Mouse Monoclonal Antibody [Clone ID: OTI9C5]

SKU CF807599

Carrier-free (BSA/glycerol-free) NDUFB11 mouse monoclonal antibody, clone OTI9C5 (formerly 9C5)

Product Data

• Clone Name: OTI9C5
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 30-163 of human NDUFB11(NP_061929) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 18.2 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B11
• Database Link:
  NP_061929
  Q9NX14
• Background: NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 [PubMed 12381726]). [supplied by OMIM, Feb 2009]
• Synonyms: CI-ESSS; ESSS; Np15; NP17.3; P17.3

Reference Data

• Protein Families: Transmembrane