HMG20A Mouse Monoclonal Antibody [Clone ID: OTI5E3]

CAT#: CF807041

Carrier-free (BSA/glycerol-free) HMG20A mouse monoclonal antibody, clone OTI5E3 (formerly 5E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A. Positive lysates LY413245 (100ug) and LC413245 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807041)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807041)

Specifications

Product Data

• Clone Name: OTI5E3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-281 of human HMG20A(NP_060670) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: high mobility group 20A
• Database Link:
  NP_060670
  Entrez Gene 66867 MouseEntrez Gene 315689 RatEntrez Gene 10363 Human
  UniProt ID Q9NP66
• Synonyms: HMGX1; HMGXB1

Reference Data

• Protein Families: Transcription Factors