PD1 (PDCD1) Mouse Monoclonal Antibody [Clone ID: OTI21F5]

CAT#: CF806927

Carrier-free (BSA/glycerol-free) PD-1 (PDCD1) mouse monoclonal antibody, clone OTI21F5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1 (1:2000). Positive lysates LY401555 (100ug) and LC401555 (20ug) can be purchased separately from OriGene.

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  PD1 ELISA with 1B11 Capture (CF807867) and 21F5 Detection (CF806927) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

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  PD1 ELISA with 7B4 Capture (CF807995) and 21F5 Detection (CF806927) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

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  Immunofluorescent staining of PDCD1 (RC210364)-stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody TA806927 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right) (1:100).

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  Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (TA806927) (Left). The rihgt is negative control. (1:100) (1:900)

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  Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody (TA806927) (Red) compared to a nonspecific negative control antibody (Blue) (1:50).

• PD1 Luminex ELISA with 1B11 Capture (CF807867) and 21F5 Detection (CF806927) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

• PD1 Luminex ELISA with 7B4 Capture (CF807995) and 21F5 Detection (CF806927) Antibodies. Substrate used: Recombinant Human PD1 (TP310364)

• Flow cytometric Analysis of stable expression PDL1 (RC213071) cells using anti-PDCD1 antibody (TA806927) (blue) or 0.3ug/ml PD1-Fc fusion protein (TP700199) (green) or both (red), and detected by anti-Fc (human) IgG-FITC (1:50).

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Specifications

Product Data

• Clone Name: OTI21F5
• Applications: ELISA, FC, IF, LMNX, Neutralize, WB
• Recommended Dilution: WB 1:2000, IF 1:100~900, FLOW 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: Homo sapiens programmed cell death 1 (PDCD1), mRNA.
• Database Link:
  NP_005009
  Entrez Gene 5133 Human
  UniProt ID Q15116
• Synonyms: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), T cell receptor signaling pathway