galectin 9 (LGALS9) Mouse Monoclonal Antibody [Clone ID: OTI2A4]

CAT#: CF805655

Carrier-free (BSA/glycerol-free) LGALS9 mouse monoclonal antibody, clone OTI2A4 (formerly 2A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS9 (RC210750, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS9. Positive lysates LY419406 (100ug) and LC419406 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LGALS9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805655)

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Specifications

Product Data

• Clone Name: OTI2A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:250
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LGALS9 (NP_002299) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.7 kDa
• Gene Name: Homo sapiens galectin 9 (LGALS9), transcript variant 2, mRNA.
• Database Link:
  NP_002299
  Entrez Gene 3965 Human
  UniProt ID O00182
• Background: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HUAT; LGALS9A