GARS1 Mouse Monoclonal Antibody [Clone ID: OTI4G4]

CAT#: CF805281

Carrier-free (BSA/glycerol-free) GARS mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Product Images

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805281)

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Specifications

Product Data

• Clone Name: OTI4G4
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GARS (NP_002038) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83 kDa
• Gene Name: Homo sapiens glycyl-tRNA synthetase 1 (GARS1), transcript variant 1, mRNA.
• Database Link:
  NP_002038
  Entrez Gene 297113 RatEntrez Gene 353172 MouseEntrez Gene 2617 Human
  UniProt ID P41250
• Background: This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. [provided by RefSeq, Jul 2008]
• Synonyms: CMT2D; DSMAV; GlyRS; HMN5; SMAD1

Reference Data

• Protein Pathways: Aminoacyl-tRNA biosynthesis