FAF1 Mouse Monoclonal Antibody [Clone ID: OTI10D1]

CAT#: CF804154

Carrier-free (BSA/glycerol-free) FAF1 mouse monoclonal antibody, clone OTI10D1 (formerly 10D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FAF1 (Cat# RC201072, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAF1(Cat# TA804154). Positive lysates LY416234 (100ug) and LC416234 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FAF1 mouse monoclonal antibody. (TA804154) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI10D1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 415-650 of human FAF1 (NP_008982) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens Fas associated factor 1 (FAF1), mRNA.
• Database Link:
  NP_008982
  Entrez Gene 14084 MouseEntrez Gene 140657 RatEntrez Gene 11124 Human
  UniProt ID Q9UNN5
• Background: Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain. [provided by RefSeq, Jul 2008]
• Synonyms: CGI-03; hFAF1; HFAF1s; UBXD12; UBXN3A

Reference Data

• Protein Families: Druggable Genome