ACE2 Mouse Monoclonal Antibody [Clone ID: OTI1G4] (Angiotensin Converting Enzyme 2)

CAT#: CF804152

Carrier-free (BSA/glycerol-free) ACE2 mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE2 (RC208442, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE2 (TA804152). (1:500)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min. (1:800)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min. (1:800)

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  Immunohistochemical staining of paraffin-embedded Human renal cell carcinoma tissue using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min. (1:800)

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  Immunohistochemical staining of paraffin-embedded carcinoma of Human lung tissue using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min. (1:800)

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  Immunohistochemical staining of paraffin-embedded Human testicular tissue within the normal limits using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min. (1:800)

• Immunohistochemical staining of paraffin-embedded Human Gastric Carcinoma using anti-ACE2 mouse monoclonal antibody (TA804152). Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min. (1:800)

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Specifications

Product Data

• Clone Name: OTI1G4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-237 of human ACE2 (NP_068576) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: angiotensin converting enzyme 2
• Database Link:
  NP_068576
  Entrez Gene 59272 Human
  UniProt ID Q9BYF1
• Background: The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
• Synonyms: ACEH

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Renin-angiotensin system