GLI1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]
CAT#: CF803967
Carrier-free (BSA/glycerol-free) GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)
Formulation: Standard
Specifications
Product Data | |
Clone Name | OTI4E2 |
Applications | IHC, WB |
Recommended Dilution | WB 1:2000, IHC 1:150 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human recombinant protein fragment corresponding to amino acids 755-1106 of human GLI1 (NP_005260) produced in E.coli. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 117.7 kDa |
Gene Name | Homo sapiens GLI family zinc finger 1 (GLI1), transcript variant 1, mRNA. |
Database Link | |
Background | This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
Synonyms | GLI; PAPA8; PPD1 |
Reference Data | |
Protein Families | Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors |
Protein Pathways | Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Antibody Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TA803967 | GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2) |
USD 478.00 |
|
TA803967AM | GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2), Biotinylated |
USD 509.00 |
|
TA803967BM | GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2), HRP conjugated |
USD 509.00 |
|
TA803967S | GLI1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2) |
USD 200.00 |
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