ISCU Mouse Monoclonal Antibody [Clone ID: OTI4F5]

CAT#: CF803397

Carrier-free (BSA/glycerol-free) ISCU mouse monoclonal antibody, clone OTI4F5 (formerly 4F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ISCU (Cat# RC212031, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ISCU(Cat# TA803397). Positive lysates LY429414 (100ug) and LC429414 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ISCU mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803397)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ISCU mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803397)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ISCU mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803397)

Specifications

Product Data

• Clone Name: OTI4F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ISCU (NP_055116) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.1 kDa
• Gene Name: iron-sulfur cluster assembly enzyme
• Database Link:
  NP_055116
  Entrez Gene 66383 MouseEntrez Gene 288740 RatEntrez Gene 23479 Human
  UniProt ID Q9H1K1
• Background: Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria. [provided by RefSeq, Jul 2008]
• Synonyms: 2310020H20Rik; HML; hnifU; ISU2; NIFU; NIFUN