DOCK8 Mouse Monoclonal Antibody [Clone ID: OTI4H10]

CAT#: CF803002

Carrier-free (BSA/glycerol-free) DOCK8 mouse monoclonal antibody, clone OTI4H10 (formerly 4H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DOCK8 (Cat# RC222789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DOCK8(Cat# TA803002). Positive lysates LY404295 (100ug) and LC404295 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DOCK8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803002)

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Specifications

Product Data

• Clone Name: OTI4H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 832-1160 of human DOCK8 (NP_982272) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 238.3 kDa
• Gene Name: Homo sapiens dedicator of cytokinesis 8 (DOCK8), transcript variant 1, mRNA.
• Database Link:
  NP_982272
  Entrez Gene 76088 MouseEntrez Gene 499337 RatEntrez Gene 81704 Human
  UniProt ID Q8NF50
• Synonyms: HEL-205; MRD2; ZIR8