Cytokeratin 7 (KRT7) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF802932

Carrier-free (BSA/glycerol-free) KRT7 mouse monoclonal antibody, clone OTI1C5 (formerly 1C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT7 (RC201124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT7. Positive lysates LY401707 (100ug) and LC401707 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-KRT7 monoclonal antibody (1:500).

• 

  Western blot analysis of A549 cell lysate (35ug) by using anti-KRT7 monoclonal antibody. Dilution: 1:500

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802932) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802932) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802932) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802932) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KRT7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802932) (1:150)

• Immunofluorescent staining of Hela cells using anti-KRT7 mouse monoclonal antibody (TA802932, green, upper left; merged, upper right) or Isotype control ( merged, lower right). Cell nuclei were stained with DAPI (blue, lower left) (1:100).

• Flow cytometric Analysis of fixed Hela cells, using anti-KRT7 antibody (TA802932), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of living OVCAR-3 cells, using anti-KRT7 antibody (TA802932), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Fixed OVCAR-3 cells, using anti-KRT7 antibody (TA802932), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

Show all 12 images

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-114 and 340-469 of human KRT7 (NP_005547) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.2 kDa
• Gene Name: keratin 7
• Database Link:
  NP_005547
  Entrez Gene 3855 Human
  UniProt ID P08729
• Background: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
• Synonyms: CK7; K2C7; K7; SCL

Reference Data

• Protein Families: ES Cell Differentiation/IPS