DDX56 Mouse Monoclonal Antibody [Clone ID: OTI2E5]

CAT#: CF802815

Carrier-free (BSA/glycerol-free) DDX56 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DDX56 (Cat# RC201477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX56(Cat# TA802815). Positive lysates LY412765 (100ug) and LC412765 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDX56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802815)

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Specifications

Product Data

• Clone Name: OTI2E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 323-547 of human DDX56 (NP_061955) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.4 kDa
• Gene Name: DEAD-box helicase 56
• Database Link:
  NP_061955
  Entrez Gene 54606 Human
  UniProt ID Q9NY93
• Background: This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
• Synonyms: DDX21; DDX26; NOH61