Topoisomerase II alpha (TOP2A) Mouse Monoclonal Antibody [Clone ID: OTI2D12]

CAT#: CF802516

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Carrier-free (BSA/glycerol-free) TOP2A mouse monoclonal antibody, clone OTI2D12 (formerly 2D12)



  View other "OTI2D12" antibodies (4)

USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI2D12
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivity Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 1100-1531 of human TOP2A(NP_001058) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 174.2 kDa
Gene Name Homo sapiens DNA topoisomerase II alpha (TOP2A), mRNA.
Background This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul
Synonyms TOP2; TOP2alpha; TOPIIA; TP2A
Reference Data
Protein Families Druggable Genome

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