USP44 Mouse Monoclonal Antibody [Clone ID: OTI1F9]

CAT#: CF801918

Carrier-free (BSA/glycerol-free) USP44 mouse monoclonal antibody, clone OTI1F9 (formerly 1F9)

Product Images

E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 420-712 of human USP44 (NP_115523) were separated by SDS-PAGE and immunoblotted with anti-USP44.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-USP44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801918)

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Specifications

Product Data

• Clone Name: OTI1F9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 420-712 of human USP44 (NP_115523) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81 kDa
• Gene Name: Homo sapiens ubiquitin specific peptidase 44 (USP44), transcript variant 1, mRNA.
• Database Link:
  NP_115523
  Entrez Gene 84101 Human
  UniProt ID Q9H0E7
• Background: Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP44 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]). [supplied by OMIM, Mar 2008]
• Synonyms: DKFZp434D0127; FLJ14528

Reference Data

• Protein Families: Druggable Genome, Protease