Galectin 3 (LGALS3) Mouse Monoclonal Antibody [Clone ID: OTI1C7]

CAT#: CF801621

Carrier-free (BSA/glycerol-free) LGALS3 mouse monoclonal antibody, clone OTI1C7 (formerly 1C7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY LGALS3 (Cat# RC208785, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3(Cat# TA801621). Positive lysates LY400834 (100ug) and LC400834 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LGALS3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA801621)

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Specifications

Product Data

• Clone Name: OTI1C7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LGALS3 (NP_002297) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens galectin 3 (LGALS3), transcript variant 1, mRNA.
• Database Link:
  NP_002297
  Entrez Gene 16854 MouseEntrez Gene 3958 Human
  UniProt ID P17931
• Background: This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
• Synonyms: CBP35; GAL3; GALBP; GALIG; L31; LGALS2; MAC2

Reference Data

• Protein Families: Secreted Protein