PPT1 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

SKU CF800618

Carrier-free (BSA/glycerol-free) PPT1 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Product Data

• Clone Name: OTI1F10
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-306 of human PPT1 (NP_000301) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 31.2 kDa
• Gene Name: palmitoyl-protein thioesterase 1
• Database Link:
  NP_000301
  P50897
• Background: The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
• Synonyms: CLN1; INCL; PPT

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways