Mitocondrial Translational Initiation Factor 3 (MTIF3) Mouse Monoclonal Antibody [Clone ID: OTI1E8]

CAT#: CF800421

Carrier-free (BSA/glycerol-free) MTIF3 mouse monoclonal antibody, clone OTI1E8 (formerly 1E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MTIF3 (Cat# RC208533, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTIF3 (Cat# TA800421). Positive lysates LY407210 (100ug) and LC407210 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MTIF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MTIF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800421)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MTIF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800421)

Specifications

Product Data

• Clone Name: OTI1E8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Dog, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 56-278 of human MTIF3 (NP_690876) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.5 kDa
• Gene Name: mitochondrial translational initiation factor 3
• Database Link:
  NP_690876
  Entrez Gene 76366 MouseEntrez Gene 477328 DogEntrez Gene 710571 MonkeyEntrez Gene 219402 Human
  UniProt ID Q9H2K0
• Background: This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5. [provided by RefSeq, Oct 2009]
• Synonyms: IF3mt