DDX59 Mouse Monoclonal Antibody [Clone ID: OTI3E9]

CAT#: CF800410

Carrier-free (BSA/glycerol-free) DDX59 mouse monoclonal antibody, clone OTI3E9 (formerly 3E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDX59 (RC207173, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDX59. Positive lysates LY422174 (100ug) and LC422174 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX59 mouse monoclonal antibody. (TA800410) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI3E9
• Applications: IHC, WB
• Recommended Dilution: WB: 1:500, IHC: 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-303 of human DDX59 (NP_001026895) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.6 kDa
• Gene Name: Homo sapiens DEAD-box helicase 59 (DDX59), transcript variant 1, mRNA.
• Database Link:
  NP_001026895
  Entrez Gene 83479 Human
  UniProt ID Q5T1V6
• Synonyms: OFD5; ZNHIT5