MAFB Mouse Monoclonal Antibody [Clone ID: OTI1E9]

CAT#: CF800290

Carrier-free (BSA/glycerol-free) MAFB mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAFB (Cat# RC207311, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAFB(Cat# TA800290). Positive lysates LY401676 (100ug) and LC401676 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MAFB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800290)

• HEK293T cells transfected with either RC207311 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAFB antibody (TA800290), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1E9
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 32-320 of human MAFB (NP_005452) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.6 kDa
• Gene Name: Homo sapiens MAF bZIP transcription factor B (MAFB), mRNA.
• Database Link:
  NP_005452
  Entrez Gene 16658 MouseEntrez Gene 54264 RatEntrez Gene 9935 Human
  UniProt ID Q9Y5Q3
• Background: The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that plays an important role in the regulation of lineage-specific hematopoiesis. The encoded nuclear protein represses ETS1-mediated transcription of erythroid-specific genes in myeloid cells. This gene contains no introns. [provided by RefSeq]
• Synonyms: DURS3; KRML; MCTO

Reference Data

• Protein Families: Druggable Genome, Transcription Factors