MIPEP Mouse Monoclonal Antibody [Clone ID: OTI5E7]

CAT#: CF800254

Carrier-free (BSA/glycerol-free) MIPEP mouse monoclonal antibody, clone OTI5E7 (formerly 5E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIPEP (RC202465, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIPEP. Positive lysates LY416973 (100ug) and LC416973 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MIPEP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800254)

• Anti-MIPEP mouse monoclonal antibody (TA800254) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIPEP (RC202465).

• HEK293T cells transfected with either RC202465 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIPEP antibody (TA800254), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5E7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 174-516 of human MIPEP (NP_005923) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.4 kDa
• Gene Name: mitochondrial intermediate peptidase
• Database Link:
  NP_005923
  Entrez Gene 81684 RatEntrez Gene 4285 Human
  UniProt ID Q99797
• Background: The product of this gene performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. This protein is primarily involved in the maturation of oxidative phosphorylation (OXPHOS)-related proteins. This gene may contribute to the functional effects of frataxin deficiency and the clinical manifestations of Friedreich ataxia. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HMIP; MIP

Reference Data

• Protein Families: Druggable Genome, Protease