TPRKB Mouse Monoclonal Antibody [Clone ID: OTI1E9]

CAT#: CF800238

Carrier-free (BSA/glycerol-free) TPRKB mouse monoclonal antibody, clone OTI1E9 (formerly 1E9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TPRKB (Cat# RC206008, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPRKB(Cat# TA800238). Positive lysates LY414223 (100ug) and LC414223 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800238)

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Specifications

Product Data

• Clone Name: OTI1E9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TPRKB (NP_057142) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.5 kDa
• Gene Name: Homo sapiens TP53RK binding protein (TPRKB), transcript variant 5, mRNA.
• Database Link:
  NP_057142
  Entrez Gene 69786 MouseEntrez Gene 51002 Human
  UniProt ID Q9Y3C4
• Synonyms: CGI-121; CGI121