RBPMS Mouse Monoclonal Antibody [Clone ID: OTI3B7]

CAT#: CF800206

Carrier-free (BSA/glycerol-free) RBPMS mouse monoclonal antibody, clone OTI3B7 (formerly 3B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RBPMS (Cat# RC200248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBPMS(Cat# TA800206). Positive lysates LY423359 (100ug) and LC423359 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RBPMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RBPMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800206)

• HEK293T cells transfected with either RC200248 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBPMS antibody (TA800206), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3B7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RBPMS (NP_001008712) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.1 kDa
• Gene Name: Homo sapiens RNA binding protein, mRNA processing factor (RBPMS), transcript variant 3, mRNA.
• Database Link:
  NP_001008712
  Entrez Gene 11030 Human
  UniProt ID Q93062
• Background: This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: HERMES

Reference Data

• Protein Families: Stem cell - Pluripotency