MYL7 Mouse Monoclonal Antibody [Clone ID: OTI4E7]

CAT#: CF800175

Carrier-free (BSA/glycerol-free) MYL7 mouse monoclonal antibody, clone OTI4E7 (formerly 4E7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MYL7 (Cat# RC206449, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL7(Cat# TA800175). Positive lysates LY412006 (100ug) and LC412006 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-MYL7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800175)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MYL7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800175)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYL7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800175)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYL7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800175)

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  HEK293T cells transfected with either RC206449 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYL7 antibody (TA800175), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYL7 (NP_067046) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.3 kDa
• Gene Name: myosin light chain 7
• Database Link:
  NP_067046
  Entrez Gene 17898 MouseEntrez Gene 289759 RatEntrez Gene 58498 Human
  UniProt ID Q01449
• Synonyms: MYL2A; MYLC2A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Tight junction