SnoN (SKIL) Mouse Monoclonal Antibody [Clone ID: OTI2F6]

SKU CF800171

Carrier-free (BSA/glycerol-free) SKIL mouse monoclonal antibody, clone OTI2F6 (formerly 2F6)

Product Data

• Clone Name: OTI2F6
• Application: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivity: Dog, Human, Monkey, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 307-684 of human SKIL (NP_005405) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 76.8 kDa
• Gene Name: SKI like proto-oncogene
• Database Link:
  NP_005405
  P12757
• Background: The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: SNO; SnoA; SnoI; SnoN

Reference Data

• Protein Families: Druggable Genome, Transcription Factors