SnoN (SKIL) Mouse Monoclonal Antibody [Clone ID: OTI2F6]

CAT#: CF800171

Carrier-free (BSA/glycerol-free) SKIL mouse monoclonal antibody, clone OTI2F6 (formerly 2F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SKIL (Cat# RC209251, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SKIL(Cat# TA800171). Positive lysates LY417307 (100ug) and LC417307 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SKIL monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SKIL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800171)

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Specifications

Product Data

• Clone Name: OTI2F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 307-684 of human SKIL (NP_005405) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 76.8 kDa
• Gene Name: SKI like proto-oncogene
• Database Link:
  NP_005405
  Entrez Gene 20482 MouseEntrez Gene 114208 RatEntrez Gene 488159 DogEntrez Gene 696680 MonkeyEntrez Gene 6498 Human
  UniProt ID P12757
• Background: The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Synonyms: SNO; SnoA; SnoI; SnoN

Reference Data

• Protein Families: Druggable Genome, Transcription Factors