TPRKB Mouse Monoclonal Antibody [Clone ID: OTI3H3]

CAT#: CF800166

Carrier-free (BSA/glycerol-free) TPRKB mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-TPRKB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TPRKB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800166)

• Anti-TPRKB mouse monoclonal antibody (TA800166) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TPRKB (RC206008).

• HEK293T cells transfected with either RC206008 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TPRKB antibody (TA800166), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3H3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TPRKB (NP_057142) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.5 kDa
• Gene Name: Homo sapiens TP53RK binding protein (TPRKB), transcript variant 5, mRNA.
• Database Link:
  NP_057142
  Entrez Gene 69786 MouseEntrez Gene 707023 MonkeyEntrez Gene 51002 Human
  UniProt ID Q9Y3C4
• Synonyms: CGI-121; CGI121