Ubiquinol Cytochrome C Reductase Core Protein I (UQCRC1) Mouse Monoclonal Antibody [Clone ID: OTI1G6]

CAT#: CF800089

Carrier-free (BSA/glycerol-free) UQCRC1 mouse monoclonal antibody, clone OTI1G6 (formerly 1G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UQCRC1 (Cat# RC203299, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UQCRC1 (Cat# TA800089). Positive lysates LY401151 (100ug) and LC401151 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UQCRC1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UQCRC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800089)

• HEK293T cells transfected with either RC203299 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UQCRC1 antibody (TA800089), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1G6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 46-305 of human UQCRC1 (NP_003356) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.5 kDa
• Gene Name: Homo sapiens ubiquinol-cytochrome c reductase core protein 1 (UQCRC1), mRNA; nuclear gene for mitochondrial product.
• Database Link:
  NP_003356
  Entrez Gene 22273 MouseEntrez Gene 301011 RatEntrez Gene 608455 DogEntrez Gene 677864 MonkeyEntrez Gene 7384 Human
  UniProt ID P31930
• Synonyms: D3S3191; QCR1; UQCR1

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease