UBE2E3 Mouse Monoclonal Antibody [Clone ID: OTI7E8]

CAT#: CF800082

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Carrier-free (BSA/glycerol-free) UBE2E3 mouse monoclonal antibody, clone OTI7E8 (formerly 7E8)



USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI7E8
Applications FC, IF, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, FLOW 1:100
Reactivities Human, Monkey, Mouse, Rat, Dog
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 22.7 kDa
Gene Name Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3), transcript variant 1, mRNA.
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Synonyms UBCH9; UbcM2
Reference Data
Protein Pathways Ubiquitin mediated proteolysis
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