UBE2E3 Mouse Monoclonal Antibody [Clone ID: OTI7E8]

CAT#: CF800082

Carrier-free (BSA/glycerol-free) UBE2E3 mouse monoclonal antibody, clone OTI7E8 (formerly 7E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBE2E3 (Cat# RC210328, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2E3(Cat# TA800082). Positive lysates LY416697 (100ug) and LC416697 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-UBE2E3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-UBE2E3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800082)

• Figure from citation: Immunofluorescence of UBE2E3 protein level by using anti-UBE2E3 antibody in HEK293E cells. Dilution: 1:150-500 View Citation

• HEK293T cells transfected with either RC210328 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-UBE2E3 antibody (TA800082), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI7E8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human UBE2E3 (NP_006348) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.7 kDa
• Gene Name: Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3), transcript variant 1, mRNA.
• Database Link:
  NP_006348
  Entrez Gene 22193 MouseEntrez Gene 295686 RatEntrez Gene 612823 DogEntrez Gene 704530 MonkeyEntrez Gene 10477 Human
  UniProt ID Q969T4
• Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
• Synonyms: UBCH9; UbcM2

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis