XTP4 (MIEN1) Mouse Monoclonal Antibody [Clone ID: OTI2D2]

CAT#: CF800021

Carrier-free (BSA/glycerol-free) C17orf37 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY C17orf37 (Cat# RC203346, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf37(Cat# TA800021). Positive lysates LY410176 (100ug) and LC410176 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-C17orf37 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-C17orf37 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800021)

• HEK293T cells transfected with either RC203346 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C17orf37 antibody (TA800021), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2D2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C17orf37 (NP_115715) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.2 kDa
• Gene Name: Homo sapiens migration and invasion enhancer 1 (MIEN1), transcript variant 1, mRNA.
• Database Link:
  NP_115715
  Entrez Gene 103742 MouseEntrez Gene 360617 RatEntrez Gene 480532 DogEntrez Gene 697837 MonkeyEntrez Gene 84299 Human
  UniProt ID Q9BRT3
• Synonyms: C17orf37; C35; ORB3; RDX12; XTP4