Necdin (NDN) Mouse Monoclonal Antibody [Clone ID: OTI5D1]

CAT#: CF506975

Carrier-free (BSA/glycerol-free) NDN mouse monoclonal antibody, clone OTI5D1 (formerly 5D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDN (Cat# RC200448, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDN(Cat# TA506975). Positive lysates LY400887 (100ug) and LC400887 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506975)

• Immunofluorescent staining of HeLa cells using anti-NDN mouse monoclonal antibody (TA506975).

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Specifications

Product Data

• Clone Name: OTI5D1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NDN(NP_002478) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.9 kDa
• Gene Name: Homo sapiens necdin, MAGE family member (NDN), mRNA.
• Database Link:
  NP_002478
  Entrez Gene 4692 Human
  UniProt ID Q99608
• Background: This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. [provided by RefSeq, Jul 2008]
• Synonyms: HsT16328; PWCR

Reference Data

• Protein Families: Druggable Genome, Transcription Factors