POLR3GL Mouse Monoclonal Antibody [Clone ID: OTI5E8]

CAT#: CF506865

Carrier-free (BSA/glycerol-free) POLR3GL mouse monoclonal antibody, clone OTI5E8 (formerly 5E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POLR3GL (Cat# RC200991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR3GL(Cat# TA506865). Positive lysates LY410247 (100ug) and LC410247 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POLR3GL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506865)

• Anti-POLR3GL mouse monoclonal antibody (TA506865) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR3GL (RC200991).

• Immunofluorescent staining of HeLa cells using anti-POLR3GL mouse monoclonal antibody (TA506865).

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Specifications

Product Data

• Clone Name: OTI5E8
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POLR3GL(NP_115681) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens RNA polymerase III subunit GL (POLR3GL), transcript variant 1, mRNA.
• Database Link:
  NP_115681
  Entrez Gene 84265 Human
  UniProt ID Q9BT43
• Synonyms: flj32422; RPC32HOM

Reference Data

• Protein Pathways: Cytosolic DNA-sensing pathway, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase