GRF2 (RAPGEF1) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF506837

Carrier-free (BSA/glycerol-free) RAPGEF1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RAPGEF1 (Cat# RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1(Cat# TA506837). Positive lysates LY404802 (100ug) and LC404802 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506837)

• Anti-RAPGEF1 mouse monoclonal antibody (TA506837) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAPGEF1 (RC208092).

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Specifications

Product Data

• Clone Name: OTI2F5
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:400~4000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 122.6 kDa
• Gene Name: Rap guanine nucleotide exchange factor 1
• Database Link:
  NP_941372
  Entrez Gene 107746 MouseEntrez Gene 63881 RatEntrez Gene 491286 DogEntrez Gene 715272 MonkeyEntrez Gene 2889 Human
  UniProt ID Q13905
• Background: This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: C3G; GRF2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Insulin signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma