CD163 Mouse Monoclonal Antibody [Clone ID: OTI2B12]

CAT#: CF506382

Carrier-free (BSA/glycerol-free) CD163 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (RC208557, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163. Positive lysates LY401362 (100ug) and LC401362 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (35ug) from 1 tissue lysate by using anti-CD163 monoclonal antibody (1:250).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA506382)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD163 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA506382) (1:500)

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  Anti-CD163 mouse monoclonal antibody (TA506382) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163 (RC208557).

• CD163 Luminex with 3C1 Capture (TA506393) and 2B12 Detection (TA506382) Antibodies. Substrate used: full length HEK293 cells expressed recombinant protein of human CD163 (TP308557).

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Specifications

Product Data

• Clone Name: OTI2B12
• Applications: IF, IHC, LMNX, WB
• Recommended Dilution: WB 1:200~4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 125.3 kDa
• Gene Name: CD163 molecule
• Database Link:
  NP_004235
  Entrez Gene 9332 Human
  UniProt ID Q86VB7
• Background: The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]
• Synonyms: M130; MM130; SCARI1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane