NY-ESO-1 (CTAG1B) Mouse Monoclonal Antibody [Clone ID: OTI4C12]

CAT#: CF505967

Carrier-free (BSA/glycerol-free) CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI4C12 (formerly 4C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B. Positive lysates LY400527 (100ug) and LC400527 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505967)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505967)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505967)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505967)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA505967)

• Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, TA505967) (1:150)

• Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CTAG1B (RC213318) using anti-CTAG1B antibody (TA505967/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

• Immunofluorescent staining of HeLa cells using anti-CTAG1B mouse monoclonal antibody (TA505967).

• Flow cytometric analysis of living NCI-H1299 cells, using anti-CTAG1B antibody (TA505967, Red), compared to an isotype control (green) (1:100).

• Flow cytometric analysis of living A375 cells, using anti-CTAG1B antibody (TA505967, Red), compared to an isotype control (green) (1:100).

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Specifications

Product Data

• Clone Name: OTI4C12
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTAG1B(NP_001318) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.8 kDa
• Gene Name: Homo sapiens cancer/testis antigen 1B (CTAG1B), mRNA.
• Database Link:
  NP_001318
  Entrez Gene 1485 Human
  UniProt ID P78358
• Background: The protein encoded by this gene is an antigen that is overexpressed in many cancers but that is also expressed in normal testis. This gene is found in a duplicated region of the X-chromosome and therefore has a neighboring gene of identical sequence. [provided by RefSeq, Jan 2012]
• Synonyms: CT6.1; CTAG; CTAG1; ESO1; LAGE-2; LAGE2B; NY-ESO-1

Reference Data

• Protein Families: Druggable Genome