ADAMTS8 Mouse Monoclonal Antibody [Clone ID: OTI2H5]

CAT#: CF505684

Carrier-free (BSA/glycerol-free) ADAMTS8 mouse monoclonal antibody, clone OTI2H5 (formerly 2H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ADAMTS8 (Cat# RC212446, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADAMTS8(Cat# TA505684). Positive lysates LY402079 (100ug) and LC402079 (20ug) can be purchased separately from OriGene.

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  Anti-ADAMTS8 mouse monoclonal antibody (TA505684) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ADAMTS8 (RC212446).

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  Immunofluorescent staining of HeLa cells using anti-ADAMTS8 mouse monoclonal antibody (TA505684).

Specifications

Product Data

• Clone Name: OTI2H5
• Applications: IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ADAMTS8(NP_008968) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.3 kDa
• Gene Name: ADAM metallopeptidase with thrombospondin type 1 motif 8
• Database Link:
  NP_008968
  Entrez Gene 11095 Human
  UniProt ID Q9UP79
• Background: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. [provided by RefSeq, Jul 2008]
• Synonyms: ADAM-TS8; METH2

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein