RHOJ Mouse Monoclonal Antibody [Clone ID: OTI1D7]

SKU CF505592

Carrier-free (BSA/glycerol-free) RHOJ mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)

Product Data

• Clone Name: OTI1D7
• Application: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 23.6 kDa
• Gene Name: ras homolog family member J
• Database Link:
  NP_065714
  Q9H4E5
• Background: ARHJ belongs to the Rho family of small GTP-binding proteins. Rho proteins regulate the dynamic assembly of cytoskeletal components for several physiologic processes, such as cell proliferation and motility and the establishment of cell polarity. They are also involved in pathophysiologic process, such as cell transformation and metastasis. [supplied by OMIM]
• Synonyms: ARHJ; RASL7B; TC10B; TCL

Reference Data

• Protein Families: Druggable Genome