TRIM44 Mouse Monoclonal Antibody [Clone ID: OTI1E10]

CAT#: CF505561

Carrier-free (BSA/glycerol-free) TRIM44 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM44 (Cat# RC201074, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM44 (Cat# TA505561)(1:500)

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  Western blot analysis of extracts (35ug) from 6 cell line lysates by using anti-TRIM44 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TRIM44 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505561)

• Anti-TRIM44 mouse monoclonal antibody (TA505561) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM44 (RC201074).

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Specifications

Product Data

• Clone Name: OTI1E10
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRIM44(NP_060053) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.3 kDa
• Gene Name: tripartite motif containing 44
• Database Link:
  NP_060053
  Entrez Gene 54765 Human
  UniProt ID Q96DX7
• Background: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, namely a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq]
• Synonyms: DIPB; HSA249128; MC7