B3GNT1 (B3GNT2) Mouse Monoclonal Antibody [Clone ID: OTI1G10]

CAT#: CF505270

Carrier-free (BSA/glycerol-free) B3GNT2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY B3GNT2 (RC208100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-B3GNT2. Positive lysates LY416552 (100ug) and LC416552 (20ug) can be purchased separately from OriGene.

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  Anti-B3GNT2 mouse monoclonal antibody (TA505270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY B3GNT2 (RC208100).

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  Immunofluorescent staining of HeLa cells using anti-B3GNT2 mouse monoclonal antibody (TA505270).

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  HEK293T cells transfected with either RC208100 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-B3GNT2 antibody (TA505270), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G10
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human B3GNT2(NP_006568) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.8 kDa
• Gene Name: Homo sapiens UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 (B3GNT2), transcript variant 1, mRNA.
• Database Link:
  NP_006568
  Entrez Gene 53625 MouseEntrez Gene 305571 RatEntrez Gene 10678 Human
  UniProt ID Q9NY97
• Background: This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It prefers the substrate of lacto-N-neotetraose, and is involved in the biosynthesis of poly-N-acetyllactosamine chains. [provided by RefSeq, Jul 2008]
• Synonyms: 3-Gn-T1; 3-Gn-T2; B3GN-T2; B3GNT; B3GNT-2; B3GNT1; beta-1; beta3Gn-T1; beta3Gn-T2; BETA3GNT; BGnT-2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Keratan sulfate biosynthesis, Metabolic pathways