SRY Mouse Monoclonal Antibody [Clone ID: OTI3C8]

CAT#: CF505267

Carrier-free (BSA/glycerol-free) SRY mouse monoclonal antibody,clone OTI3C8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SRY (Cat# RC210382, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SRY(Cat# TA505267). Positive lysates LY418872 (100ug) and LC418872 (20ug) can be purchased separately from OriGene.

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  Anti-SRY mouse monoclonal antibody (TA505267) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRY (RC210382).

Specifications

Product Data

• Clone Name: OTI3C8
• Applications: IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SRY(NP_003131) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.7 kDa
• Gene Name: sex determining region Y
• Database Link:
  NP_003131
  Entrez Gene 6736 Human
  UniProt ID Q05066
• Background: This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome. [provided by RefSeq, Jul 2008]
• Synonyms: SRXX1; SRXY1; TDF; TDY