MRPS34 Mouse Monoclonal Antibody [Clone ID: OTI4H2]

CAT#: CF505229

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Carrier-free (BSA/glycerol-free) MRPS34 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)



USD 600.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI4H2
Applications IF, IHC, WB
Recommended Dilution WB 1:500~2000, IHC 1:150, IF 1:100
Reactivities Human, Mouse, Rat
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 25.5 kDa
Gene Name Homo sapiens mitochondrial ribosomal protein S34 (MRPS34), transcript variant 2, mRNA; nuclear gene for mitochondrial product.
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
Synonyms MRP-S12; MRP-S34; MRPS12
Reference Data
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