NDUFB10 Mouse Monoclonal Antibody [Clone ID: OTI4F1]

CAT#: CF505207

Carrier-free (BSA/glycerol-free) NDUFB10 mouse monoclonal antibody, clone OTI4F1 (formerly 4F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB10 (RC200526, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB10 (TA505207). Positive lysates LY417917 (100ug) and LC417917 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NDUFB10 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505207)

• Anti-NDUFB10 mouse monoclonal antibody (TA505207) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDUFB10 (RC200526).

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Specifications

Product Data

• Clone Name: OTI4F1
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NDUFB10(NP_004539) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.6 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B10
• Database Link:
  NP_004539
  Entrez Gene 68342 MouseEntrez Gene 681418 RatEntrez Gene 479887 DogEntrez Gene 693482 MonkeyEntrez Gene 4716 Human
  UniProt ID O96000
• Synonyms: PDSW

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease