NDUFB10 Mouse Monoclonal Antibody [Clone ID: OTI1H6]

CAT#: CF505113

Carrier-free (BSA/glycerol-free) NDUFB10 mouse monoclonal antibody, clone OTI1H6 (formerly 1H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NDUFB10 (Cat# RC200526, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB10(Cat# TA505113). Positive lysates LY417917 (100ug) and LC417917 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NDUFB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505113)

• HEK293T cells transfected with either RC200526 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDUFB10 antibody (TA505113), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1H6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NDUFB10(NP_004539) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.6 kDa
• Gene Name: Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.
• Database Link:
  NP_004539
  Entrez Gene 4716 Human
  UniProt ID O96000
• Synonyms: PDSW

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease