TSC22D1 Mouse Monoclonal Antibody [Clone ID: OTI4H8]

CAT#: CF505062

Carrier-free (BSA/glycerol-free) TSC22D1 mouse monoclonal antibody, clone OTI4H8 (formerly 4H8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TSC22D1 (Cat# RC223210, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TSC22D1(Cat# TA505062). Positive lysates LY416882 (100ug) and LC416882 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TSC22D1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA505062)

• Flow cytometric Analysis of Jurkat cells, using anti-TSC22D1 antibody (TA505062), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Hela cells, using anti-TSC22D1 antibody (TA505062), (Red), compared to a nonspecific negative control antibody, (Blue).

• Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-TSC22D1 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

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Specifications

Product Data

• Clone Name: OTI4H8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TSC22D1(NP_006013) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.5 kDa
• Gene Name: TSC22 domain family member 1
• Database Link:
  NP_006013
  Entrez Gene 21807 MouseEntrez Gene 498545 RatEntrez Gene 8848 Human
  UniProt ID Q15714
• Background: TSC22D1 encodes a transcription factor and belongs to the large family of early response genes. [supplied by OMIM]
• Synonyms: Ptg-2; TGFB1I4; TSC22

Reference Data

• Protein Families: Transcription Factors