ACBD3 Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF504892

Carrier-free (BSA/glycerol-free) ACBD3 mouse monoclonal antibody, clone OTI1B5 (formerly 1B5)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ACBD3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA504892)

• HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACBD3 (RC208434, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACBD3. Positive lysates LY411600 (100ug) and LC411600 (20ug) can be purchased separately from OriGene.

• Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ACBD3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• HEK293T cells transfected with either RC208434 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACBD3 antibody (TA504892), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-ACBD3 antibody (TA504892), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1B5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ACBD3(NP_073572) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 60.4 kDa
• Gene Name: acyl-CoA binding domain containing 3
• Database Link:
  NP_073572
  Entrez Gene 170760 MouseEntrez Gene 289312 RatEntrez Gene 699311 MonkeyEntrez Gene 64746 Human
  UniProt ID Q9H3P7
• Background: The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in the maintenance of Golgi structure and function through its interaction with the integral membrane protein giantin. It may also be involved in the hormonal regulation of steroid formation. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: GCP60; GOCAP1; GOLPH1; PAP7

Reference Data

• Protein Families: Druggable Genome