CD31 (PECAM1) Mouse Monoclonal Antibody [Clone ID: OTI1C2]

CAT#: CF504862

Carrier-free (BSA/glycerol-free) PECAM1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECAM1 (RC208654, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECAM1. Positive lysates LY424716 (100ug) and LC424716 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-PECAM1 monoclonal antibody at 1:200.

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  Western blot analysis of extracts (35ug) from human kidney tissue by using anti-PECAM1 monoclonal antibody. 1:500

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PECAM1 mouse monoclonal antibody. (TA504862; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunofluorescent staining of 293T cells transiently transfected by pCMV6-ENTRY PECAM1 (RC208654) using anti-PECAM1 mouse monoclonal antibody (TA504862, green, upper left; merged, upper right). Cell nuclei were stained with DAPI (blue, lower left). 293T cells transfected with empty vector plasmid served as negative control ( merged, lower right) (1:100).

• Living HEK293T cells transfected with either RC208654 plasmid (red) or empty vector (blue) were immunostained by anti-PECAM1 antibody (TA504862) or isotype control antibody (green), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living HUVEC cells, using anti-PECAM1 antibody (TA504862), (Red), compared to a nonspecific negative control antibody, (green) and PBS (blue).

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Specifications

Product Data

• Clone Name: OTI1C2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150, FLOW 1:50
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PECAM1(NP_000433) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: Homo sapiens platelet and endothelial cell adhesion molecule 1 (PECAM1)
• Database Link:
  NP_000433
  Entrez Gene 5175 Human
  UniProt ID P16284
• Background: The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May
• Synonyms: CD31; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration