DPH2 Mouse Monoclonal Antibody [Clone ID: OTI2B12]

CAT#: CF504856

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Carrier-free (BSA/glycerol-free) DPH2 mouse monoclonal antibody, clone OTI2B12 (formerly 2B12)



  View other "OTI2B12" antibodies (4)

USD 545.00


Availability*
In Stock

Size
    • 100 ug


Specifications

Product Data
Clone Name OTI2B12
Applications FC, IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150, FLOW 1:100
Reactivity Human, Rat
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Full length human recombinant protein of human DPH2(NP_001375) produced in HEK293T cell.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 51.9 kDa
Gene Name Homo sapiens diphthamide biosynthesis 2 (DPH2), transcript variant 1, mRNA.
Background This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Synonyms DPH2L2
Reference Data

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